Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
نویسندگان
چکیده
منابع مشابه
FED-BATCH BIOREACTOR PROCESS WITH RECOMBINANT Saccharomyces cerevisiae GROWING ON CHEESE WHEY
Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify β-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and β-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best ...
متن کاملFed-batch cultivation of Saccharomyces cerevisiae in a hyperbaric bioreactor.
Fed-batch is the dominating mode of operation in high-cell-density cultures of Saccharomyces cerevisae in processes such as the production of baker's yeast and recombinant proteins, where the high oxygen demand of these cultures makes its supply an important and difficult task. The aim of this work was to study the use of hyperbaric air for oxygen mass transfer improvement on S. cerevisiae fed-...
متن کاملFed-Batch Bioreactor with Mortality Rate
We address the problem of finding an optimal feedback control for feeding a fed-batch bioreactor with one species and one substrate, from a given initial condition to a given target value in a minimal amount of time. Mortality rate for the biomass and nutrient recycling are taken into account in this work. The optimal synthesis (optimal feeding strategy) has been obtained by Moreno in 1999 when...
متن کاملAdaptive Control of Saccharomyces cerevisiae Yeasts Fed-Batch Cultivations
Fed-batch baker’s yeast cultivation is a complex biotechnological process from the viewpoint of measurement and control. It is a non-linear system with not well-known dynamics. The process is non-stationary due to metabolic changes, modifications in cell physiology and multiple increases in biomass concentration over the cultivation time. Furthermore, there is lack of cheap and reliable online ...
متن کاملKey process conditions for production of C(4) dicarboxylic acids in bioreactor batch cultures of an engineered Saccharomyces cerevisiae strain.
A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter(-1) of malate at a yield of 0.42 mol (mol glucose)(-1) in calcium carbonate-buffered shake flask cultures. With shake flasks, process parameters that are important for scaling up this process cannot be controlled independently. In thi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Chemical Engineering
سال: 2006
ISSN: 0104-6632
DOI: 10.1590/s0104-66322006000400001